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Dr Vincent Postis


About Dr Vincent Postis

Vincent trained as a biochemist, and has developed research interests with multidisciplinary approaches ranging from molecular biology to biophysics. Vincent’s research output on membrane protein study of biomedical relevance have been published in high-profile international peer reviewed journals. Over the past 15 years, Vincent has been focusing his research on functional and structural characterisation of membrane transporter from organisms ranging from bacteria to humans. He has had a major contribution in developing techniques to improve membrane protein production and crystallisation. During his PhD, Dr Postis' studies focused on the adenine nucleoside transporter (Ancp) in yeast.

In 2005 he joined the University of Leeds as a Post-doctoral Research Fellow within the Membrane Protein Structure initiative (MPSi). He greatly contributed to the success of this project by designing and implementing automated methods for the high throughput cloning, expression and characterisation of membrane proteins. These methods led to the successful 2D and 3D crystallisation of multiple membrane proteins.

In 2010, he studied a peroxisomal ABC transporter (CTS) in insect cells known to be responsible for X-linked adrenoleukodystrophy and helped understanding that CTS has an additional thioesterase activity which is coupled to substrate transport and ATPase activities.

Current Teaching

Vincent teaches the following modules on our BSc Biomedical Sciences courses:

  • Biochemistry and Molecular Biology
  • Clinical Biochemistry

And the following modules on out MSc Toxicological Sciences, MSc Microbiology and Biotechnology, and MSc Applied Biomedical Sciences Research:

  • Bioanalytical Techniques
  • Research Project

Research Interests

Today, Vincent's research focuses on a wide range of transporters of biomedical interest but a main aim is the study of the structure/function relationship of nucleoside transporters. His interest is focused on nucleoside transport into cells.

Using a range of biophysical approaches, the research program that Vincent is involved in aims to understand the conformational changes undergone by the nucleoside transporters during substrate transport across the lipid membrane, i.e. to probe the dynamics of the proteins in addition to determining the static structures of individual conformations by X-ray and electron crystallography.

Selected Publications

Journal articles (16)

  • Yamashita M; Shepherd M; Booth WI; Xie H; Postis V; Nyathi Y; Tzokov SB; Poole RK; Baldwin SA; Bullough PA (2014), Structure and function of the bacterial heterodimeric ABC transporter CydDC: Stimulation of atpase activity by thiol and heme compounds. Journal of Biological Chemistry, vol. 289
    https://doi.org/10.1074/jbc.M114.590414
    View Repository Record
  • Lousa CDM; van Roermund CWT; Postis VLG; Dietrich D; Kerr ID; Wanders RJA; Baldwin SA; Baker A; Theodoulou FL (2013), Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids. Proceedings of the National Academy of Sciences, vol. 110
  • Huysmans GHM; Chan N; Baldwin JM; Postis VLG; Tzokov SB; Deacon SE; Yao SYM; Young JD; McPherson MJ; Bullough PA (2012), A urea channel from Bacillus cereus reveals a novel hexameric structure. Biochemical Journal, vol. 445 (2), p. 157-166.
    https://doi.org/10.1042/BJ20120169
  • Vaziri H; Baldwin SA; Baldwin JM; Adams DG; Young JD; Postis VLG (2012), Use of molecular modelling to probe the mechanism of the nucleoside transporter NupG. Molecular membrane biology, vol. 30
    View Repository Record
  • Glover CAP; Postis VLG; Charalambous K; Tzokov SB; Booth WI; Deacon SE; Wallace BA; Baldwin SA; Bullough PA (2011), AcrB contamination in 2-D crystallization of membrane proteins: lessons from a sodium channel and a putative monovalent cation/proton antiporter. Journal of structural biology, vol. 176
  • Newstead S; Drew D; Cameron AD; Postis VLG; Xia X; Fowler PW; Ingram JC; Carpenter EP; Sansom MSP; McPherson MJ (2011), Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2. The EMBO journal, vol. 30
  • Troshin PV; Postis VLG; Ashworth D; Baldwin SA; McPherson MJ; Barton GJ (2011), PIMS sequencing extension: a laboratory information management system for DNA sequencing facilities. BMC research notes, vol. 4
    View Repository Record
  • Gutmann DAP; Mizohata E; Newstead S; Ferrandon S; Henderson PJF; Van Veen HW; Byrne B (2009), A high-throughput method for membrane protein solubility screening: The ultracentrifugation dispersity sedimentation assay. Protein science, vol. 16
  • Xia X; Xia X; Postis VLG; Rahman M; Xia X; Postis VLG; Rahman M; Wright GSA; Roach PCJ; Deacon SE (2008), Investigation of the structure and function of a Shewanella oneidensis arsenical-resistance family transporter. Molecular membrane biology, vol. 25
  • Deacon SE; Roach PCJ; Postis VLG; Deacon SE; Roach PCJ; Postis VLG; Wright GSA; Xia X; Deacon SE; Roach PCJ (2008), Reliable scale-up of membrane protein over-expression by bacterial auto-induction: from microwell plates to pilot scale fermentations. Molecular membrane biology, vol. 25
  • Roach PCJ; Postis VLG; Deacon SE; Roach PCJ; Postis VLG; Deacon SE; Wright GSA; Ingram JC; Xia X; Roach PCJ (2008), Large-scale preparation of bacterial cell membranes by tangential flow filtration. Molecular membrane biology, vol. 25
  • Postis VLG; Deacon SE; Postis VLG; Deacon SE; Roach PCJ; Wright GSA; Xia X; Postis VLG; Deacon SE; Roach PCJ (2008), A high-throughput assay of membrane protein stability. Molecular membrane biology, vol. 25
  • Gutmann DAP; Mizohata E; Newstead S; Ferrandon S; Postis V; Xia X; Henderson PJF; Van Veen HW; Byrne B (2007), A high-throughput method for membrane protein solubility screening: The ultracentrifugation dispersity sedimentation assay (vol 16, pg 1422, 2007). PROTEIN SCIENCE, vol. 16
  • Postis V; De Marcos Lousa C; Arnou B; Lauquin GJ-M; Trézéguet V (2005), Subunits of the yeast mitochondrial ADP/ATP carrier: cooperation within the dimer. Biochemistry, vol. 44
  • De Marcos Lousa C; Trézéguet V; David C; Postis V; Arnou B; Pebay-Peyroula E; Brandolin G; Lauquin GJ-M (2005), Valine 181 is critical for the nucleotide exchange activity of human mitochondrial ADP/ATP carriers in yeast. Biochemistry, vol. 44
  • Zeman I; Schwimmer C; Postis V; Brandolin G; David C; Trézéguet V; Lauquin GJ-M (2003), Four mutations in transmembrane domains of the mitochondrial ADP/ATP carrier increase resistance to bongkrekic acid. Journal of bioenergetics and biomembranes, vol. 35
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