Leeds Beckett University - City Campus,
Woodhouse Lane,
LS1 3HE
Amino Acid Profiling via LC-MS/MS
Targeted analysis of amino acids is challenging and requires specialist expertise to accurately quantify low-abundance metabolites in human blood samples.
Targeted analysis of amino acids is challenging and requires specialist expertise to accurately quantify low-abundance metabolites in human blood samples. Many routine laboratory approaches lack the specificity, sensitivity, and multiplex capability required for confident amino acid profiling, whereas liquid chromatography tandem mass spectrometry (LC-MS/MS) provides robust, targeted quantification across multiple analytes within a single run.
The Carnegie School of Sport at Leeds Beckett University, delivered through the Centre for Active Lifestyles and Healthy Ageing (ACTLIFE), offers a professionally delivered LC-MS/MS amino acid profiling service, providing targeted analysis of 15 amino acids in human plasma or serum samples. Amino acid profiles are key indicators of metabolic and physiological status and can provide valuable insights to support decision-making in clinical pathways, applied performance environments, and structured research activity.
The service currently offers robust and validated quantification of 15 amino acids, with scope to discuss additional analytes or expanded panels where scientifically and operationally appropriate. Workflows are delivered by experienced academic and technical staff, supported by validated methods, defined quality control procedures, and transparent reporting.
This service is offered as a standalone analytical service and can also support academic or methodological collaboration where appropriate, depending on user needs.
- High-specificity LC-MS/MS quantification of free amino acids
- Simultaneous measurement of multiple analytes within a single assay
- Clear quality control and assay performance reporting
- Access to experienced academic and technical leads without obligation to collaborate
- Suitable for clinical assessment, applied performance monitoring, service evaluation, and research
By providing details about your research using the enquiry form below, our in-house experts will contact you with a quote and clear instructions on how to submit your samples to us.
Then the analysis will be conducted by our in-house team, and you will and you will receive a structured data report including amino acid concentrations, calibration performance, and quality control outcomes. Turnaround times will be agreed in advance to support applied and clinical timelines.
This service is available to clinical services, applied performance environments, academic institutions, and industry partners. We analyse human plasma or serum samples only, from any cohort, subject to appropriate governance and approvals.
We offer three tiers of service, aligned to different use cases and levels of analytical assurance. Costs vary by tier and sample number.
- Standard (Grade I pricing). Single preparation and single injection per sample, with quality control samples analysed every ten study samples. Suitable for routine monitoring, service evaluation, and exploratory use.
- Comprehensive I (Grade II pricing). Single preparation with duplicate injections per sample alongside quality controls. Appropriate where increased confidence is required for applied or clinical interpretation.
- Comprehensive II (Grade III pricing). Duplicate sample preparation with duplicate injections for each preparation, alongside quality controls. Designed for high-stakes clinical decision support or externally scrutinised outputs.
A minimum of 20 samples is normally required per submission.
If you are interested in using this service, complete the enquiry form below. To receive an accurate quote, please indicate your intended use (clinical, applied, performance, or research), preferred service tier, and number of samples, and whether you wish to discuss additional analytes beyond the current amino acid panel (e.g., vitamin D3, creatine).
Name:
Organisation:
Email:
Phone:
Request:
featured experts
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![Dr Theocharis Ispoglou staff profile image]()
Professor Theocharis Ispoglou
Director of the Centre for Active Lifestyles and Healthy Ageing (ACTLIFE) and Professor of Exercise and Nutrition Sciences at Leeds Beckett University. Provides strategic and scientific leadership across the service and is central to partner engagement, collaboration design, and governance, with expertise in muscle health, nutrition, and applied physiology. -
Dr Luke Aldrich
Dr Aldrich has over four years of hands-on LC–MS/MS experience, including extensive manufacturer-led training with Shimadzu. He has co-authored peer-reviewed publications in amino acid analysis with Professor Ispoglou and has led method optimisation, validation, and routine service delivery across multiple projects. -
Professor John O’Hara
Professor John O'Hara is the Director of the Centre for Human Performance. He is an applied scientist with expertise in carbohydrate metabolism, endurance performance, environmental physiology and nutrition. John is an accredited sport scientist with the British Association of Sport and Exercise Sciences. He is a highly experienced applied sport scientist who has worked with amateur and professional athletes across sporting disciplines for many years.
featured experts
-
![Dr Theocharis Ispoglou staff profile image]()
Professor Theocharis Ispoglou
Director of the Centre for Active Lifestyles and Healthy Ageing (ACTLIFE) and Professor of Exercise and Nutrition Sciences at Leeds Beckett University. Provides strategic and scientific leadership across the service and is central to partner engagement, collaboration design, and governance, with expertise in muscle health, nutrition, and applied physiology. -
Dr Luke Aldrich
Dr Aldrich has over four years of hands-on LC–MS/MS experience, including extensive manufacturer-led training with Shimadzu. He has co-authored peer-reviewed publications in amino acid analysis with Professor Ispoglou and has led method optimisation, validation, and routine service delivery across multiple projects. -
Professor John O’Hara
Professor John O'Hara is the Director of the Centre for Human Performance. He is an applied scientist with expertise in carbohydrate metabolism, endurance performance, environmental physiology and nutrition. John is an accredited sport scientist with the British Association of Sport and Exercise Sciences. He is a highly experienced applied sport scientist who has worked with amateur and professional athletes across sporting disciplines for many years.
