Leeds Beckett University - City Campus,
Woodhouse Lane,
LS1 3HE
Centre for Active Lifestyles and Healthy Ageing (ACTLIFE)
High-intensity intermittent, or resistance, or moderate-continuous exercise effects on endocrine systems - Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Previous research has compared hormonal responses to exercise using parallel designs and analytical techniques that are not robust enough minimise random variability perhaps leading to conflicting findings. Therefore, we aimed to this project is the first study to simultaneously explore 16 hormonal responses to exercise using a randomised crossover design utilising LC-MS/MS.
By understanding hormonal responses to exercise, optimal training interventions can be created to improve health and performance.
Ten adult males completed 30 min of moderate-intensity continuous exercise (<65% VO2), high-intensity interval exercise (>80% VO2), and whole-body resistance exercise in a randomised crossover design. Blood pressure, heart rate, RPE, and blood samples were collected pre-, immediately post-, and 2 h post-exercise. Blood samples were analysed for glucose, lactate and 16 steroid hormones were profiled, which were simultaneously analysed via LC-MS/MS.
Project is currently ongoing but may lead to:
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